FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n01_atac_root_rep2_1.351000000ea27a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n01_atac_root_rep2_1.351000000ea27a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31083947
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC310800.029.5885245
CTCCGAG790600.028.21575745
TCTCCGA1306800.017.77790345
ATCTCCG1633550.014.2273744
ACTCCTA15600.013.55744131
CATCTCC1853300.012.56225443
GTGCTAG10900.011.771121
ACATCTC1976200.011.749104542
GTCTTAG7350.011.637661
GTATAGA15800.011.3973081
GTGTAAG14250.011.3733141
TACACCG88350.011.3074895
GGTCTAG8950.011.0662141
TCTAGAC7050.010.8512633
CACATCT2155100.010.77181541
GTGTAGA44650.010.5868621
CTCCTAT23450.010.45832932
GTCCTAC23300.010.3370661
GTATAGG18800.010.2969831
GTGTAGG45000.010.204391
Produced by FastQC (version 0.11.2)