FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n01_atac_root_rep1_1.351000000ea251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n01_atac_root_rep1_1.351000000ea251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26431386
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG433750.027.2921145
TCCGAGC183050.025.90020245
TCTCCGA719950.017.51788345
ATCTCCG895500.014.10513444
GTCCTAG7900.012.5368741
GTGTAAG14400.012.5052151
GTCTTAG8100.012.5052151
CATCTCC1030450.012.27318343
GTCCTAC22200.011.6602671
ACATCTC1086300.011.61108542
TACACTG13600.011.5809145
TTACACT9600.011.48440554
GTGTTAG9250.011.43720151
GTCTTAC12200.011.2546941
GTCTAAG8250.011.1864821
GTGCTAG12000.011.0671151
GTGTAGG40500.011.0045881
GTGTTAC14600.010.9463461
TACACCG82000.010.9207625
CTACACT12850.010.856064
Produced by FastQC (version 0.11.2)