FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n02_atac_root_rep3_1.352000000ea284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n02_atac_root_rep3_1.352000000ea284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27667731
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC582150.022.7194845
TGACGCT260150.020.24275645
TCTGACG780300.018.84207245
GTCCTAG7400.015.1982821
ATCTGAC975850.015.08419744
CATCTGA1102650.013.339375543
GTGTAAG14850.012.7235721
ACATCTG1237900.011.94167942
TACACCG90200.011.7456025
GTGCTAG12600.011.6037671
GTACTAG7500.011.3966851
GTATAAG13500.010.9968021
GTCCTAC24050.010.9427631
CACATCT1353900.010.93671941
GGATAAG23700.010.9145471
GTATAGA14900.010.8693231
GGTCTAG8500.010.8497861
GTCTAAG8300.010.8402211
GTGTTAG9000.010.7468741
GTGTTAT12400.010.7025331