FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n02_atac_root_rep2_2.352000000ea2ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n02_atac_root_rep2_2.352000000ea2ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23989294
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC534050.023.86828845
TGACGCT226750.020.41582545
TCTGACG723650.018.6782745
GTGTTAG8700.015.2542821
ATCTGAC903250.014.97597744
CATCTGA1024100.013.19114243
GTTCTAG9850.012.7882411
TACACTG11850.012.528165
GTGTAGA39200.012.5091971
GTGTAAG14200.012.1972351
GTCTTAG7300.012.0171311
TTACACT9250.011.915534
TACACCG69200.011.7669575
ACATCTG1156900.011.75265242
GTACTAG6350.011.68958951
GTGCTAC21800.011.4531641
GTCTAGC8900.011.3731691
GTCCTAG7350.011.323311
TCTACAC36500.011.2159873
GTCCTAA7650.010.8792581
Produced by FastQC (version 0.11.2)