FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n02_atac_root_rep2_1.352000000ea26a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n02_atac_root_rep2_1.352000000ea26a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30299230
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT359350.025.62675945
CTGACGC871050.024.92699245
TCTGACG1242600.018.34471345
ATCTGAC1535900.014.842504544
CATCTGA1725950.013.1898743
GTCCTAG7400.012.158731
CTAGACT6250.011.8767074
ACATCTG1931350.011.81834742
GTCTTAG7400.011.5507941
GTATAGA16000.011.5279951
TACACCG82500.011.3423655
GTGTAGA45850.011.3326791
GTCTAGA7350.011.3233341
GTGTAAG13550.011.2883271
TAGACTG9350.011.0665045
GTTCTAG11000.011.0423371
CACATCT2085400.010.99278141
GTCCTAC24450.010.855831
GTCTAGG10000.010.7969511
GTGTTAG8650.010.4016891
Produced by FastQC (version 0.11.2)