FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n02_atac_root_rep1_1.352000000ea241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n02_atac_root_rep1_1.352000000ea241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25744788
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC521950.021.87818145
TGACGCT240900.019.8243245
TCTGACG673250.018.33872445
ATCTGAC836200.014.77256344
GTCCTAG8000.013.7772891
CTACACT11750.013.2089694
CATCTGA942750.013.09580243
GTACTAG7400.013.0705661
GTGTAGG41650.012.5294121
TAGGACT11200.012.2509434
TAGACTG9650.011.6547255
ACATCTG1067800.011.62930242
GTGTTAG8800.011.5023751
GTGTAAG13700.011.4930471
GTCCTAC23000.011.2467671
TACACCG78250.011.1533875
CACATCT1169350.010.64042241
TACACTG14200.010.6131885
GTTCTAG10250.010.5335571
GTCTTAG6450.010.4621091
Produced by FastQC (version 0.11.2)