FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n01_atac_root_rep3_1.351000000ea287.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n01_atac_root_rep3_1.351000000ea287.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27667731
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG481950.027.25017545
TCCGAGC200400.025.73347945
TCTCCGA812550.018.20930345
ATCTCCG1024100.014.43260744
CATCTCC1165800.012.76521343
ACATCTC1229750.012.07625442
TATACTG11800.011.6330145
GTCTAGG9150.011.563791
GTGTAGA39950.011.4393851
GTCTTAC14000.011.4170461
TACACCG88700.011.2642885
TCTACAC37050.011.2364193
GTGTAAG14300.011.1775271
GTCCTAC22900.011.1087811
GGGTTAC27000.011.0894831
CACATCT1350100.010.97809241
GTGTTAC15650.010.932581
GTCCTAG7700.010.8176881
CTACACT11750.010.7249324
CTTAGAC7400.010.6434093
Produced by FastQC (version 0.11.2)