FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n01_atac_root_rep2_2.351000000ea2a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n01_atac_root_rep2_2.351000000ea2a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23989294
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG451000.028.04209945
TCCGAGC181850.025.21531145
TCTCCGA754350.017.952545
ATCTCCG944850.014.37379944
GTGTAAG12350.012.7603781
CATCTCC1084600.012.60057643
GTGTTAG8300.012.4770571
ACATCTC1146750.011.869184542
TACACCG68800.011.8403425
GTCTAGA8050.011.4662241
GTATAGG15150.010.9964251
CTAGGAC6200.010.8886393
CACATCT1255850.010.8398641
GTGTAGG37450.010.8206431
GTGTTAC14400.010.78745651
GTCTAAG8150.010.7730681
GTACAGA17250.010.7018091
GTGCTAG10150.010.6465191
GTCCTAC20550.010.6265511
GTCCTAA7000.010.6132491
Produced by FastQC (version 0.11.2)