FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n01_atac_root_rep2_1.351000000ea26d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n01_atac_root_rep2_1.351000000ea26d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30299230
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC303100.030.45717445
CTCCGAG770200.027.9623245
TCTCCGA1281650.017.87641145
ATCTCCG1593900.014.36065344
ACTCCTA15250.013.13052231
CATCTCC1800100.012.764443
CTAGACT6700.012.0913314
GTCCTAG8400.012.0601621
ACATCTC1920750.011.93255342
GTGCTAG11800.011.4469331
TACACCG82550.011.3129915
CACATCT2088750.010.9717341
GTCTAGA7650.010.8883031
GTGTTAC13500.010.8392561
TAGGACT12250.010.8383294
GTGTAGA42900.010.5477221
GTACTAG5200.010.3902941
GGGTAAG22850.010.3448221
GTGTAGG46400.010.33431151
TATACTG10500.010.2872285
Produced by FastQC (version 0.11.2)