FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l01n01_atac_root_rep1_1.351000000ea244.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l01n01_atac_root_rep1_1.351000000ea244.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25744788
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG426600.026.60269445
TCCGAGC184600.025.43678345
TCTCCGA699250.017.63272745
ATCTCCG871950.014.10974944
GTGCTAG11600.013.002681
GTCCTAC25250.012.3928021
CATCTCC1003350.012.37404343
ACATCTC1057500.011.7175442
GTCTAAG8250.010.9149591
CTAAGAC6650.010.8286833
TACACTG13200.010.7402395
GTGTTAG7550.010.7342481
GTACAGA17500.010.677171
TACACCG81300.010.6288935
CACATCT1166300.010.62831141
ACTAGAC6150.010.611343
TCTACAC38200.010.6036773
GTGTAGA40000.010.5806891
TAGACAG9400.010.5334865
GTCCTAG8150.010.4964411
Produced by FastQC (version 0.11.2)