FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17597618
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2249091.278065019936221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA32600.040.23209868
GCCGTAT36650.039.4127169
CCGTATC29400.037.94993270
GCGCTAT20350.036.8524065
CCGCTAG12100.033.30655
CGCTAGA19800.032.7355046
CGCTATA26100.032.619576
GAGCGCT18200.032.577213
AGCGCTC20350.031.7066174
AGCGCTA17800.031.3237444
GCGCTCA13000.030.7310945
TCGCCGT43500.030.31080467
CGCTATT30900.030.0469156
CCGCTAT27000.029.8524915
AGTGTGC35300.029.83034938
CCAGATC65250.029.1379818
GCGCTAA17550.027.955515
GTGCCTC33500.027.6688441
GCGCTAG13400.027.460035
TAGCGCT13900.025.9968663
Produced by FastQC (version 0.11.5)