FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ZZ_sample_mixup_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ZZ_sample_mixup_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42878034
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT70400.042.405135
GGCGCTA52450.041.6370664
GCGCTAG38900.034.8179635
AGCGCTA58250.033.045154
GGGCGCT32700.032.215573
GGCGCTC44800.031.9511384
CGCGCTA38800.031.9310024
CGCTATA93000.030.93376
CGCTATT113650.030.4249976
CGCTAGT59000.029.8372676
GCGCTCA46650.029.633815
GCGCTCT62700.028.7462835
CCGCTAT97500.028.536755
AGCGCTC58150.027.8056644
GCGCTAA53300.027.5124235
CCGCTAG47750.026.6057385
CGCCGTA63900.026.51226268
CGGCGCT42800.026.3305053
TGCGCTA49250.026.2217184
CAGCGCT47650.026.0008453
Produced by FastQC (version 0.11.5)