FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_TGA5_Ler06Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_TGA5_Ler06Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283075
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4530.16002826106155613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGA150.002223167370.035
CGGTCCT1200.070.03
GGCGATA150.002223167370.018
GGTCCTA4550.067.692314
ACGGTCC850.065.8823552
GTCCTAG2400.065.6255
GTCCTAT6700.064.253735
AGTCCTA5950.064.1176454
TCCTAGA4750.062.631586
GTCCTAA5600.062.5000045
TCCTATA7300.062.3287666
TCCTAGG1800.062.2222256
CGGCTCC453.8380676E-1062.2222252
AGGTCCT3550.062.112683
CCTATCG850.061.7647067
GCTCCTA3400.061.7647064
GTGTCCT2550.061.76473
TGTCCTA4450.061.3483124
ATCCTAT10100.061.3366285
AAGGTCC1600.061.252
Produced by FastQC (version 0.11.5)