FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_TGA5_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_TGA5_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890017
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTC1206.548362E-1132.08109714
GCTGCCC450.004308232631.11068736
TCCGGGG604.9854355E-429.16463315
ACGCCGC1451.8189894E-1128.96675140
GCCGTTA1352.582965E-1028.5197345
GTCCGCA500.00720998927.99961920
GGGGTAC500.007211966427.9980459
GAACGCC1358.083589E-925.92557138
CGTCCTA700.001226836624.9982554
CGGAAGT1701.4006218E-1024.70693262
CCGTTAG1601.8626451E-924.06352446
GTCCTAT1854.0381565E-1022.7011185
CACGGAG800.002667759321.87347217
AGCCGTC1807.2614057E-921.38979753
GTGGGGT1807.2705006E-921.38739612
TAGAGCC2001.0677468E-921.00089350
GCCGTCG1859.955329E-920.81169554
TGTGGGG1859.968062E-920.80935911
AGAGCCG1901.3531462E-820.2640251
CGCCGTT2101.9608706E-920.0008544
Produced by FastQC (version 0.11.5)