FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_TGA5_Col10Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_TGA5_Col10Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40199
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21135.256349660439314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCC150.00220925670.000012
GGGTTCC150.00220925670.000012
GCCTCCA600.070.000013
ATGACTC150.00220925670.000011
CTCCACG150.00220925670.000015
ACACTCC150.00220925670.000012
CACTGCA150.00220925670.000018
CGTAGAA150.00220925670.0000110
GACATCC150.00220925670.000012
TGCCTCC150.00220925670.000012
CCATCTT150.00220925670.000017
CACTCGG150.00220925670.000018
CACTTGG150.00220925670.000018
CACTCAG150.00220925670.000018
TGGACGG150.00220925670.0000121
GCGTCCA150.00220925670.000013
GGGATCC150.00220925670.000012
CTTGTAA150.00220925670.0000110
CACTAGC150.00220925670.000018
CACTAAA309.993164E-870.000018
Produced by FastQC (version 0.11.5)