FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_TGA5_Col09Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_TGA5_Col09Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14226
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG470.3303809925488542No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG150.002179803870.000012
ACACGGA150.002179803870.000013
ACCGGAC600.070.000014
ACTAAAC150.002179803870.000019
AACCGGA309.5333235E-870.000013
AGGACTG150.002179803870.000016
TGCCGGA150.002179803870.000013
GGACTGT1000.070.000017
GGACTGG309.5333235E-870.000017
CCACGGA150.002179803870.000013
GGACTCG150.002179803870.000017
GGACTCA150.002179803870.000017
GGACTAA401.200533E-1070.000017
TACGGAC700.070.000014
GACGGAC252.6990892E-670.000014
CGGACTA1400.070.000016
ATGCGGA252.6990892E-670.000013
ACTCTCT252.6990892E-670.000019
ACTTCTT252.6990892E-670.000019
GCGGACT1200.070.000015
Produced by FastQC (version 0.11.5)