FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_TGA5_Col05Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_TGA5_Col05Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211710
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4310.2035803693731992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACT150.002222450569.9995333
GTGCGGC150.002222450569.9995348
GGTAATC2450.059.999594
CGGTAAT1650.059.3935363
GGGTAAT2500.058.7996033
CCGGGTA252.3838633E-456.012851
CGTGTGG252.3866483E-455.99962237
GCGTTAA407.4433046E-752.5120472
AGCCGGA200.0069444352.4996563
TGGCGCC200.0069444352.4996558
CCGGACA200.0069444352.4996518
AGTGCGG200.0069444352.4996547
ACGGACA200.0069444352.499657
AGTTCCG200.0069444352.4996555
TGAGGGT200.0069444352.4996534
GGCGCCA200.0069444352.4996559
GCGTAAT1150.051.738783
GGGGTAA950.051.5907822
GTAATCT3800.051.5785985
CGCTAAT552.2719178E-950.9087453
Produced by FastQC (version 0.11.5)