FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_TGA5_2019poolH_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_TGA5_2019poolH_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452296
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11180.2471832605196597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGC150.002224796269.993912
GGCGCTA3800.066.30274
AGGTCCT6450.065.653213
GCGCTAT4500.065.327665
GGTCCTA6950.064.95124
CGCTATT5900.064.662556
GTCCTAT11100.064.6340265
GCGCTCT2400.064.161095
CGCTCAT2350.064.0440756
GAGTCCT7350.063.8039783
CAGGCGC550.063.6308332
TCCTATA16450.063.6185266
CCGCTAT5300.063.3907245
ACGCGCT1050.063.327833
CGCGCTC1050.063.320834
CGGTCGC501.4551915E-1162.9945222
GGCGCTC2500.062.9875644
TCGCTAG2450.062.8516855
GTCGCTA2450.062.8447344
GTCCTAG4850.062.778055
Produced by FastQC (version 0.11.5)