FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_LHY1_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_LHY1_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046460
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14850.1419070007453701No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGCC200.00694865652.50612666
CGCTGCG200.006951282752.5011143
CCGGTAT1301.05657506E-418.84835466
GCGGGTT1400.003753093315.00031832
TCAGGGT1600.009094086513.125277534
ACCGGGT2000.00278068412.25025835
TGTAGGG6908.058123E-109.63834670
CCAAGAG3700.00301414298.51450765
GTGTAGG7553.8207872E-88.34494669
TAGATCG11102.0008883E-117.88342669
GTCGTGT9806.863047E-97.50087666
CGTCGTG9806.863047E-97.50087665
CGTGTAG8502.8091017E-77.4126368
TCGTGTA10051.1161319E-87.314286767
AGGATCT4800.00378597787.29112510
GCGTCGT10452.3630491E-87.034313764
AGAGTGT5150.007488646.796584669
AGCGTCG11351.139706E-76.476526763
AGATCGG26550.06.064448470
GAGCGTC12355.574475E-75.95182762
Produced by FastQC (version 0.11.5)