FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_LHY1_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_LHY1_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226003
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28740.12911033812622894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG900.005277348719.44510742
CCCAGTG1000.00970127217.49745215
TGTCGCA1750.001017345814.002048570
CGTGTAG13807.2759576E-127.10248970
CGTCGTG17350.06.85979370
GGAAGCA6300.00103816726.665695716
GCGTCGT18400.06.46833869
GAGCGTC21800.06.42295870
TCGTGTA16953.092282E-116.19559769
AGCGTCG20050.06.1106268
AGAGCGT24250.06.062742769
GTAGGGA11103.500778E-65.991867569
GTGTAGG15101.2522105E-75.56372869
GTCGTGT16402.130946E-85.549592568
CGGAAGA36000.05.44524170
TCGGAAG36350.05.20021169
TGTAGGG12159.357573E-55.18594468
AAGAGCG28000.05.1257568
TAGGGAA14601.2070286E-55.03498370
Produced by FastQC (version 0.11.5)