FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_LHY1_Col_UB_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_LHY1_Col_UB_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330575
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224600.6743580312708767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT52450.064.258913
GGTAATC64200.063.452834
GGGTAAT59300.062.797093
GCGTAAT27450.062.3475073
GTGGTCG180900.061.39546270
GTAATAG71450.061.128735
CGATAAT48900.060.979423
GGTAATA111200.060.7729764
GTAATCT97100.060.7674985
AGGTAAT85650.060.272223
AGTAATC82750.059.886414
GTAATAT167550.059.759435
GTAATCA78900.059.3044665
CGGGTAA16050.058.8772932
GATAATC69500.058.8154034
TGGTAAT89000.058.7900433
GGTAATG43700.058.3019334
GAGTAAT82350.058.1400073
GGCTAAT30950.058.123943
GTAATAC49150.058.0319445
Produced by FastQC (version 0.11.5)