FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_LHY1_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_LHY1_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393997
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31510.226040658624086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT21450.065.1021963
GGTAATC21050.063.6767854
GCGTAAT12700.063.658773
GGGTAAT26350.063.090363
GGTAATA54150.062.303584
GCGCGTA1200.061.2562451
AGTAATC30350.060.8849074
CCGTAAT12100.060.741283
GTAATAG28600.060.5722355
GAGTAAT30400.060.4415743
AGGTAAT34250.060.1872833
GTAATCC13000.060.033825
GTGGTCG13950.059.46629370
GTAATAT91450.059.317445
GTAATCT38250.058.7405555
CGTAATA27500.058.4136624
GCGGTAA6000.058.33512
GCCTAAT11150.057.755453
GTAATCA28950.057.059495
CGGGTAA8100.057.0387652
Produced by FastQC (version 0.11.5)