FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_LHY1_Col16cycle_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_LHY1_Col16cycle_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6820310
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG54800.06.387361570
GTGTAGG39300.06.32365170
CGTGTAG35100.06.282543769
GTAGGGA29200.06.11349270
GAGCGTC60150.06.11020470
CGTCGTG47650.05.87664169
GTCGTGT46100.05.84644570
TGTAGGG31400.05.796632369
TCGTGTA46200.05.606499768
AGGCTAC7500.007298925.60027555
AGAGCGT70200.05.43489970
GCGTCGT51000.05.421993370
AAGAGCG78800.05.108268369
GCTTCGT12551.5609089E-45.020019546
Produced by FastQC (version 0.11.5)