FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_LHY1_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_LHY1_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341393
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39450.2940972556141265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGC4450.069.995712
GGCGCTC30650.069.31064
CGGCGCT29550.069.2850953
CGCCGCT22850.069.22993
CCCGCTA44800.069.136394
GGGCGCT32550.069.135553
AGGGCGC7900.069.1096952
GCCCGCT18900.069.069843
CCGCTAG33050.069.042675
TCGCTAT81250.069.0050055
AGGCGCT37700.068.9745563
CGCTATA119900.068.97416
CGGGCGC9900.068.935172
CCCCGCT25050.068.878023
CGCTATT119400.068.823266
CGCTCAT37400.068.779216
GGCGCTA74700.068.777584
CGACGCT16900.068.753193
GCCGCTC19600.068.745794
TGCGCTA43550.068.7099154
Produced by FastQC (version 0.11.5)