FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_Col_input_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_Col_input_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57413167
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG690950.06.403232670
GTGTAGG659700.06.351063770
TGTAGGG601600.06.16150770
GTAGGGA539050.06.0583369
GCGTCGT1086750.06.022970770
GTCGTGT922650.05.93333170
TAGGGAA576300.05.763970
AGCGTCG1180950.05.7233470
CGTCGTG1002200.05.7068570
TCGTGTA865350.05.65073569
GAGCGTC1281050.05.6231370
GGGAAAG486050.05.365073769
CGGAAGC124350.05.235594370
AAGAGCG1591050.05.218307570
CAGTACG68900.05.181789470
AGAGCGT1430000.05.15246370
GAAGAGC1865850.05.05006770