FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColSPL9_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColSPL9_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208987
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17180.14210243782604776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGC850.003792783520.5864492
GGCGGCC850.003792783520.58644932
CGCGAAT950.007204104718.42402567
CCCCGAT1200.001341159817.49992845
ACGAGCG1250.001761250716.80201550
CGGAGGT1550.00737289113.54889242
GTCGGTG1550.00737289113.54889239
GGTTAGC2305.7087094E-412.17537458
CATAGCC2950.0045430779.49266461
TATTGTC6300.007570376.110580421
GTGTAGG13751.7345883E-86.109823770
GAGCGTC24650.05.964204370
GTAGGGA11405.4922402E-65.83403370
CGTCGTG20452.1827873E-115.64859970
AGAGCGT27400.05.621112370
TGTAGGG12756.1474293E-65.490854769
GCGTCGT20401.200533E-105.490854769
CGTGTAG14754.5702836E-75.458281569
GTCGTGT18106.188202E-95.415238467
AGAGTGT8550.00522335675.32249668
Produced by FastQC (version 0.11.5)