FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColSPL9_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColSPL9_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336312
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4170.12399200742167987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTC200.00694206252.51093339
ACGCGGG200.00695023452.49531631
GTGCATG459.1767506E-538.89698839
GGTCACG450.004299785531.1175959
TCCTGAC500.00721160327.99333815
CTAATCG800.002664642421.87304733
CATGTGC1003.9240762E-421.00124736
GGACTAT850.003787229320.586427
GTTCCAC900.00526744519.44560236
CCTTAAC950.007210153618.4166712
CCCAAGC950.007210153618.416672
CTCAGTG1000.00968981117.49583620
TGTGCAT1503.1493924E-416.33430538
GAGTTAC1300.002289907316.15000310
AAGACTG1350.002945476715.5518553
CTTCTGA1656.4928946E-414.85157851
CACCATT2000.002779322212.2470865
CGTGTAG4602.5030931E-69.89336470
TAGGGAA3702.8547077E-49.46142969
AGGGAAA4451.8182618E-59.44016870
Produced by FastQC (version 0.11.5)