FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColSPL9_Col_UB_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColSPL9_Col_UB_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306481
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14830.4838799142524333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGTA150.00222266570.005391
GCGTTAA500.070.005392
GGGCGTA150.00222266570.005391
AGCGCTC150.00222410469.99396532
GCGGTAA850.065.887422
GTGGTCG23850.064.1349170
GTAATCG1300.061.927845
GTAATAC3150.061.115815
CGGTAAT3150.061.115813
CGTAATG1150.060.8742524
GTAATCC2950.060.5131265
GGTAATC4750.060.4256974
GTAATCA5250.060.004625
GCGTAAT1750.060.0046163
GGGTAAT4550.060.0046123
CCGTAAT2300.059.3523943
AGTAATC4800.059.0670434
CCTTAAT3150.058.8934173
GCGCGTA308.36168E-658.337821
CGCTAAT1200.058.337823
Produced by FastQC (version 0.11.5)