FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColSPL9_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColSPL9_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201893
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7450.36900734547507835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGCG252.8325103E-670.0000170
AGCCGGT150.00222224170.027
CTCGTGC150.00222224170.062
CCTAGGG150.00222224170.046
GCCGGTA150.00222224170.01
GTAGCAC150.00222224170.017
GGTAATA5150.061.8446584
CCGGTAA1250.061.62
ACCGGTA800.061.251
GGGTAAT3200.060.156253
TAATACC1750.060.06
AGGTAAT4000.059.5000043
GATAATC3650.059.4520534
GCGTAAT1300.059.2307743
CGGTAAT3250.059.230773
GTAATAG3750.058.8000035
CTGGGTA308.355193E-658.3333321
GTGGTCC308.355193E-658.33333270
GGCTAAT1200.058.3333323
GGTAATC3100.057.5806474
Produced by FastQC (version 0.11.5)