FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColSPL9_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColSPL9_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785628
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46450.5912467478246702No Hit
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTAT24320.3095612681829059Illumina Single End PCR Primer 1 (96% over 33bp)
AGCGGCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTAT20600.26221061367466536Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC3250.068.925712
CGGCGCT12900.068.641663
CCGCTCA14950.068.5935755
GTCCGCT12250.068.569693
GGCGCTC16950.068.3463444
AGGCGCT18200.068.2674943
GACGCTA14100.068.260674
ACGCTAT19050.068.1609955
GACCGCT9300.068.116553
GCCCGCT9100.068.075193
CCCGCTA18800.067.950394
CGCTATT47700.067.943766
GGGCGCT13550.067.931853
GTGCGCT8300.067.889833
CGCTATC24550.067.859786
CGCTAGA24500.067.8554156
CGCTAAT29200.067.840746
GCGCTAG19250.067.816455
CGCTAGT18750.067.758286
AAGGCGC4600.067.719982
Produced by FastQC (version 0.11.5)