FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColCAMTA1_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColCAMTA1_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273801
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53370.4189822429092142No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT22250.063.3882453
GGTAATC26000.062.4516944
GTAATCT39050.061.206745
GTAATAG28150.060.9140245
GGGTAAT23150.060.7727363
GTAATCC15250.060.1216745
AGGTAAT32250.060.0107573
GGTAATA44700.059.655034
GCGTAAT13300.059.2058453
GTAATAT63800.058.9640585
AGTAATC30500.058.8595774
CGCTAAT7750.058.7050323
GTGGTCG13300.058.42790270
CGTAATC11950.058.2753334
CGATAAT17050.058.089253
CCGTAAT11800.057.5378233
GTAATCA30300.057.2847025
GATAATC29650.057.1242144
GGTAATT27850.057.0466654
GAGTAAT29400.056.900263
Produced by FastQC (version 0.11.5)