FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColCAMTA1_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColCAMTA1_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562789
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27150.48241881060219727No Hit
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGG22780.4047698160411806Illumina Single End PCR Primer 1 (97% over 35bp)
AGCGGCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGG17080.30348851878768063Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG401.3460522E-1070.01
CCCGTCC150.002224312970.060
AGGTGCG301.02334525E-770.01
CTGGCGG150.002224312970.030
GGCGCTA20100.069.651744
GGGCGCT9300.069.2473143
CCGCTAA12350.069.1497965
GCCGCTC7800.069.102564
CCGCTAT23000.068.9347845
CGGCGCT9750.068.923073
GACCGCT6400.068.906253
GCCGCTA12200.068.852464
CCCGCTA11450.068.777294
CGCTATT30300.068.729386
CGACGCT5500.068.727273
TGCGCTA10550.068.672994
CGCTAAT18150.068.45736
CAGGCGC4300.068.372092
CCGCTAG10700.068.364495
GCGCTAT27750.068.360365
Produced by FastQC (version 0.11.5)