FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColCAMTA1_Col_UB_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColCAMTA1_Col_UB_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8906104
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144890.16268617568355365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG18850.08.5419281
GGTCGCC8001.0220174E-77.87570656
CGCCTGT12604.9112714E-117.223032570
TCTTCGC37700.06.685099670
GCCTGTG16159.094947E-126.502277469
TCGCCTG16202.6666385E-95.83388967
GTAGGGA123750.05.77034470
GTCTTCG40050.05.76843769
CGCCGTA7300.0054121055.753843359
GCGCGTC7950.00221553825.723912269
GTGTAGG148600.05.629837570
GAGTGTC51700.05.619585569
CGTGTAG155950.05.61140270
GTCGTGT205600.05.550233470
TGTAGGG136000.05.45649469
AGCGTCG252600.05.36283470
CGTCGTG219600.05.33985269
AGCGCGT9250.00228021365.297831568
CTTCGCC26701.8189894E-125.112932770
TCGTGTA191150.05.109126669
Produced by FastQC (version 0.11.5)