FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColCAMTA1_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColCAMTA1_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135104
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31250.14636289379814754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAG1450.004743417714.48195519
ACTCGCC2253.037073E-513.99922323
TCCGGCA2254.7540152E-412.44375330
TGGTCGC2050.00334707611.95167556
CCGGAGG2200.00567307811.13600635
CCTGACC2903.2488356E-410.86146527
CCGTGTG3000.0052273469.33303414
GGTGGCA3450.00159951289.13078358
ACAGCCT3150.00780059688.88922847
AGTCTGG3700.0030208498.5132412
GTTCCCT3850.0043219018.1821341
ATCACCC3850.00432555058.18136328
TTACGTG5108.19651E-47.549484761
GACGTGT4850.004191017.21626313
TACGTGT6552.1281632E-46.94699362
ACGATTG5150.00750348676.79589937
GAGTGTA10309.749492E-76.456557370
CCTTATG6100.00541713286.311272635
CCTCAAT6200.00641011356.20991453
TCTCGTC6250.00696557256.160379463
Produced by FastQC (version 0.11.5)