FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_ColCAMTA1_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_ColCAMTA1_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587028
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7200.12265173041149655No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA200.006948912552.50274769
GCGCCCT200.006948912552.50274769
CGCCGTA350.001254943840.00209459
GTCGCAG601.2150982E-535.00183551
TGCGCCC400.00241886234.99884868
TCGCGAG400.00241886234.99884867
GTCGGGG400.00241886234.99884818
AGCCGCC604.9823144E-429.16570735
CTGGCAC500.007205728.00146548
TTGCGCC500.007208697527.99907967
CGCACGA500.007208697527.99907931
GGTCGCC700.001225465625.00130856
AGTCGCA851.3074891E-424.70717650
GTCGCCG901.9266251E-423.33455557
TACGCTA750.00183231723.3325674
CGCTAGC750.00183231723.3325678
CGCTGTT850.003787438220.58931455
CACTGGC850.003787438220.58931456
GTTACGG850.003787438220.58931448
ACCTCGA900.005274852319.44380626
Produced by FastQC (version 0.11.5)