FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24SPL9_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24SPL9_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603931
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8960.1483613194222519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGG200.00694921352.50235762
GAGCGCT200.00694921352.50235752
CGAGCGC350.001255017940.001851
CGTCCGT652.1029216E-532.3064740
GACCGTC552.9924497E-431.81697737
TCGGCGC450.00430765631.10993435
CGACCGT604.98263E-429.1655636
TCCCGTC500.007209035627.9989425
CCGTCCG700.001226150924.99905439
ACCGTCC952.7800005E-422.10442738
GTCCGTT800.002666281821.87417241
ACGACCG850.003789543220.58745635
AGGGGGG1107.4526155E-419.0980931
GTGCTGA950.00720486918.4218866
GCGATTA1000.00968731817.49933641
TACCTGA1400.003752118214.9994329
GTAGGAG1450.004737640714.4822144
AATGAGC2050.003344209211.95076753
GTAGAAT2250.00669215510.88937848
CCAAGCT2800.00295132359.99962114
Produced by FastQC (version 0.11.5)