FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24SPL9_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24SPL9_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279571
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4550.16274935526216955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG150.00222221570.0073356
GTCGGAC150.002223792569.9948130
CCCCTGA200.00694368552.505565
GGTCTGC200.00694368552.505553
TGCCGGG200.00694368552.505557
GCACGCA200.006948599652.496119
TCGTCAC200.006948599652.4961112
GGGCCGA200.006948599652.496115
CCGCGGT200.006948599652.4961134
CTCCGCA305.8738753E-446.66320424
TCGGCTC400.00241719234.99740648
AGGTAAC450.004304499431.10880533
TACCGGA450.004304499431.10880523
GCGAAAG500.007197499328.00293443
ACCGCTA500.007203788527.99792530
GGAAACG500.007203788527.99792545
CCCTCAA657.939333E-426.92589665
TTAAGCC700.001223485625.00261942
GTCCTTA800.002663295721.87337922
AAACGAC800.002663295721.87337919