FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24SPL9_Col_B_gnew-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24SPL9_Col_B_gnew-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034049
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTGC10400.09.42374570
GTGTGCC7051.1336488E-78.44031470
GTAGGGA30450.06.782089770
AGCGTCG60550.06.647869670
TCCTTCG7652.0209147E-46.404838613
TGTAGGG33650.06.241258669
GTGCCTC6200.00641006466.21011868
GAGCGTC65350.06.05255669
GAGTGTG16304.9112714E-106.012795469
GTGTAGG35600.05.997616368
AAGAGCG80950.05.794110370
GTCGTGT48400.05.785534470
CGTGTAG37250.05.7319567
CGTCGTG53250.05.58734369
TTTGGCG7600.0084459325.526343339
GGGAAAG29300.05.4952767
AGAGCGT72800.05.38499768
TCGTGTA45800.05.34972470
GCGTCGT55550.05.292904468
GAAGAGC93900.05.069665469
Produced by FastQC (version 0.11.5)