FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24SPL9_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24SPL9_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638964
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25090.39266687951120877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT4800.064.1665953
CGGTAAT10550.061.7060933
GTGGTCG21500.061.20923270
GTAATAT31250.060.3679355
CCGTAAT6000.060.0832673
AGCGCTA352.9722833E-760.004631
GTAATCT17450.059.9712835
GGGTAAT12200.059.6720623
GGTAATA22350.059.5077634
GGTAATC9650.059.4817964
CGTAATA9900.059.040344
CGTAATC5500.058.545394
GTAATAG15200.058.256515
AGTAATC15400.058.1817554
CGCGTAA1700.057.6469962
GTAATAC8800.057.2726675
CGATAAT9200.057.0651553
AGGTAAT15100.057.0198023
GTAATCA14000.056.999945
CGTTAAT5500.056.63633
Produced by FastQC (version 0.11.5)