FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24SPL9_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24SPL9_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182560
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7780.4261612620508326No Hit
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA5700.3122261174408414Illumina Single End PCR Primer 1 (96% over 33bp)
AGCGGCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA4520.24758983347940405Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGC150.002217932470.03152567
ACACCCG301.020162E-769.993161
CACGCTA950.069.993164
AAGGTCG150.002222760669.9931622
GCGCAGC150.002222760669.993165
CGTAGCG301.020162E-769.993161
CCGAGGG150.002222760669.9931629
GGAGTAC301.020162E-769.993165
GCAGGCG150.002222760669.993161
CAGTACG150.002222760669.993161
CGGAGTA301.020162E-769.993164
AGGGACG150.002222760669.993161
ACTGCCG252.8330687E-669.993151
CGTGCCG207.914622E-569.993151
GGTCCTA353.6925485E-969.993154
CACGCTC401.3460522E-1069.993154
GCGCCAG750.069.993155
CCGCTTA550.069.993155
CGACAGA207.914622E-569.993156
CCGCTAC1350.069.993155
Produced by FastQC (version 0.11.5)