FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24CAMTA1_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24CAMTA1_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790318
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28670.3627653678645811No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT6100.063.1120153
GGTAATC13850.062.1633724
CGGTAAT12000.061.830653
GTAATCT19550.061.2249645
GGGCGTA650.059.2281951
GGTAATA26000.058.958984
AGGTAAT18900.058.3308033
CGCTAAT4150.058.1902473
GTAATAT36000.058.1363685
GGGTAAT14000.057.7474943
GTAATCG6550.057.707425
GTAATAG17350.057.4903035
AGTAATC17600.057.071394
CGTAATC6700.056.9378284
GGTAATG9950.056.6307074
GATAATC16550.056.4628034
CGATAAT11200.056.247563
TGGTAAT18900.056.1086773
CTAATAG9600.055.7788285
CCGTAAT7850.055.7300643
Produced by FastQC (version 0.11.5)