FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24CAMTA1_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24CAMTA1_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649231
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28180.4340519784175432No Hit
AGCGCCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTG24850.38276052745478883Illumina Single End PCR Primer 1 (97% over 34bp)
AGCGGCGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTG17150.2641586738772486Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGC2000.070.000622
GTCACGC700.070.000622
AGGGCGC3800.070.000612
GGCGCTA25450.069.72024
CCCGCTA15150.068.840194
GCCGCTC9000.068.8286364
CCGCTAT29700.068.816865
CCGCTGT8650.068.781435
GGGCGCT11050.068.728353
CCGCTCA13350.068.684455
GCGCTAT34250.068.666855
ACTGCGC2550.068.628062
CGCTATG15250.068.618276
TGGCGCT15100.068.604593
CGGCGCT12450.068.58973
CCCCGCT9400.068.505973
CGCTATT38950.068.467736
CCCGCTC8750.068.395334
TCGCTAT27800.068.358645
GGCGCTC14900.068.351044
Produced by FastQC (version 0.11.5)