FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24CAMTA1_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24CAMTA1_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170536
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32020.147521165278991No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA1000.009695874517.4991194
GCCCTCG1350.002948722615.55477328
CGGCGCA1550.00737627813.54864145
CCGTCCC2852.0312025E-512.28149970
TCCCCGA2250.00670636310.8883426
CCAGTCC2903.245267E-410.86277365
GGGCATA2750.00254652910.18130610
TCGCTCA2950.0045476059.49214155
CTCGGTC3200.0088777898.74996237
TAAGCGT3600.00235720728.7495594
TACGGCC4350.00144069858.0463142
GTAGGGA16000.07.219218367
TCACAGG5000.0056303757.00045455
TCCGTCC5050.0062007426.93114369
CAGTCAT5050.0062007426.93114354
CCCGAAA5750.00291783276.696086462
CTCACGA6300.00103565546.66709966
GCATATC5800.00319918156.637596612
TGTAGGG18700.06.364048566
TAGGGAA18950.06.280090368
Produced by FastQC (version 0.11.5)