FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n02_C24CAMTA1_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n02_C24CAMTA1_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5525508
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72850.13184308121533803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG10950.08.6303121
CCGACGT4800.00379200027.2911552
TGGTCGC5904.907124E-47.119175455
CTAACGC6056.5380055E-46.942666564
CCCTGCG6107.182075E-46.88569860
CAGTCCG7156.2011066E-46.364053768
CGGCATG7206.7454367E-46.31997356
TAGGGGT6650.00191248716.31528853
CGTCGTG64250.05.829281370
GTAGGGA36050.05.631539370
AAGAGCG97650.05.556020370
GCTAACG8850.00130633425.53713663
TCGGTGG9505.339415E-45.52667851
GCGTCGT69250.05.50948669
TTAGGGG7700.009763335.4541622
CTCGGCA8600.00560979555.2911470
CCGGCCG8600.00561993245.290326623
AGCGTCG72300.05.276971368
GAGCGTC77900.05.257179370
GTGGTCG8850.0078454275.141626454
Produced by FastQC (version 0.11.5)