FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17597618
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1888911.0733895917049683No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206150.11714653653693358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGA58000.059.171496
GCGCTAG33000.057.6705365
GCGCTAT23100.049.825655
CCGCTAG26650.049.7520525
GGGCGCT23000.049.5859683
GCTAGAT65900.045.832547
GAGCGCT22300.045.0242083
CGCTATA30000.043.3803676
GCGCTCA18250.042.3641435
AGCGCTC19850.040.711824
CCGCTAT27500.039.9453285
CGCGCTA12300.038.6814584
AGCGCTA19200.036.6238374
CGCTATT33900.035.190566
AGCGCTG11250.035.139454
ACGCTAG14650.034.6257675
CGCTAGT14650.034.387166
GTGCGCT12850.033.7588463
TCGCTAG17000.033.3376585
TGCGCTA14500.033.2950864
Produced by FastQC (version 0.11.5)