FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ZZ_sample_mixup_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ZZ_sample_mixup_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42878034
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA67900.053.868574
GGGCGCT51750.050.2536243
GCGCTAG49750.047.489725
GCGCTAT66150.046.0868725
CGCTAGA104750.043.071386
GAGCGCT54300.040.8675273
GCCGCTA74350.039.5916824
AGCGCTA56500.038.842584
CCGCTAG60800.037.074155
GCGCTAA55850.037.0384865
GGCCGCT48350.036.5581173
GGCGCTC40500.036.3844644
CGCTATA91900.035.0398526
GCGCTCA39650.034.780995
CGGCGCT41800.034.0805853
AGCGCTC43650.034.079534
GGAGCGC27850.033.18032
GCGCGCT32850.032.2846573
CGCGCTA32300.031.7507534
CGCTATT108000.031.3071336
Produced by FastQC (version 0.11.5)