FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_TGA5_input_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_TGA5_input_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11715100
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14550.012.74907650
TATGCCG14150.012.1200848
ATGCCGT16100.011.73907149
GCCGTCT14400.010.45133651
CGTATGC17900.09.77648646
GTATGCC18700.09.17107747
CTCGTAT25350.06.627185344
GTCACCT33700.06.439136529
TCGTATG32600.05.69015545
CCGTCTT32000.05.57809752
GCTATCT32400.05.40120739
CACCTGA43200.05.34719531
GCACACG74200.05.330161670
AGTCACC38150.05.32107428
TATCTCG38450.05.00647741
Produced by FastQC (version 0.11.5)