FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_TGA5_Ler06Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_TGA5_Ler06Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283075
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCC455.456968E-1270.000012
GTGCTCG150.002223167370.067
CGTGTGC150.002223167370.064
GGGTCCT5900.069.406783
TCCTAGG1650.067.878796
CGGTCCT2450.067.142863
GGTCCTA7300.067.123284
GTGTCCT4700.067.021283
GGGTGTC950.066.3157961
GTCCTAT8050.065.6521765
CTGTCCT1500.065.3333363
GGTGTCC2250.065.3333362
AGGGGTC1350.064.814811
GGTCCTG1850.064.3243264
GCGGTCC1200.064.1666642
TCCTGGC600.064.1666646
TGTCCTA5250.064.04
GTCCTGT2300.063.913045
GGCTCCT2850.063.859653
TGTCCTC2700.063.5185174
Produced by FastQC (version 0.11.5)