FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_TGA5_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_TGA5_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890017
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCCG450.004308080331.1109122
GAGGCCC450.004308080331.1109170
GGCTAGT1401.2732926E-1129.99980752
ACTTCCG1700.028.82334368
CACGGCG1606.548362E-1126.24983243
ACACGGC1659.640644E-1125.45438242
CACTGGG700.001226383424.9998416
GGCGATG2300.024.34766846
CGGGAGT1202.1670894E-623.3344963
GTCTCGC1802.8558134E-1023.3331837
CGATGGC1854.0381565E-1022.70255748
CACACGG1703.756213E-922.64691441
CCGTTAA2207.2759576E-1222.27258517
CTTCCGA2056.002665E-1122.1949869
TTCCGAC1905.638867E-1022.10512270
TGGCTAG2201.5643309E-1020.68168651
GGGTCCT1558.740517E-720.3235913
GGGAGTA1408.244271E-620.0009964
GCTAGTA2101.9626896E-919.99987253
CGGCGAT2302.8376235E-1019.7824845
Produced by FastQC (version 0.11.5)