FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_TGA5_Col10Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_TGA5_Col10Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40199
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTC150.00220925670.000011
ATAAGTC150.00220925670.000011
TATACCA150.00220925670.0000161
TATATCC150.00220925670.000012
TGTCCAC600.070.000014
GTCGTCC150.00220925670.000012
CACGTCC150.00220925670.000012
AGGGTCC150.00220925670.000012
GGATTAC150.00220925670.0000115
CACTGTC150.00220925670.000018
GTCTGAC150.00220925670.0000147
CACTGGA150.00220925670.000018
CACTGCT150.00220925670.000018
CACTGAG150.00220925670.000018
CCCATCC150.00220925670.000012
TCTATCC150.00220925670.000012
CCACTGA309.993164E-870.000017
CCCCGTC150.00220925670.000011
ACTTATG150.00220925670.000019
TATCCAC1550.070.000014
Produced by FastQC (version 0.11.5)